巻号一覧
38 巻, 8 号
選択された号の論文の66件中51～66を表示しています
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大和 進, 杉原 久義, 嶋田 健次1990 年 38 巻 8 号 p. 2290-2292
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーAn alternative method for the assay of chloramphenicol using an enzymatic reaction coupled with a fluorescence detection system has been developed. Chloramphenicol was enzymatically acetylated by chloramphenicol acetyl-transferase in the presence of acetyl-coenzyme A (acetyl-CoA) as the acetyl-donor, after which the liberated CoA-SH was derivatized with a fluorogenic reagent, 4-(aminosulfonyl)7-fluoro-2, 1, 3-benzoxadiazole. The assay was linear over the range of 2.5-40 μg/ml. Analytical recoveries of chloramphenicol at concentrations of 7.5 and 22.5 μg/ml, added to human serum, plasma, or a slightly hemolyzed serum, were in the range of 93.3 to 106.2%. The enzymatic assay was not affected by the presence of ten other antibiotics tested.抄録全体を表示PDF形式でダウンロード (491K) -
梅香家 佳彦, 能田 均, 季 明求, 大倉 洋甫1990 年 38 巻 8 号 p. 2293-2295
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーmeso- and dl-1, 2-diarylethylenediamines (14 species) were evaluated for pre-column fluorescence derivatization reagents in the high-performance liquid chromatographic determination of catecholamines (norepinephrine, epinephrine and dopamine) in human urine and plasma. Of the compounds, meso-1, 2-bis(4-methoxyphenyl)ethylenediamine was most preferable for all the catecholamines in terms of sensitivity and selectivity. The detection limit for each catecholamine is approximately 0.5 fmol in a 50-μl injection volume.抄録全体を表示PDF形式でダウンロード (475K) -
稲森 善彦, 網野 比佐子, 坪井 万里子, 山口 智美, 辻坊 裕1990 年 38 巻 8 号 p. 2296-2298
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーRacemomycin-B (RM-B), the main component of Streptomyces lavendulae OP-2 which is the basis of 50% of the antibiotics produced, is a streptothricin antibiotic which contains three β-lysine moieties in the molecule. RM-B had antimicrobial activity against plant-pathogenic microorganisms and growth-inhibitory activity against the root of Brassica rapa L. at the concentration of 50 ppm. It strongly inhibited the growth of Pseudomonas syringae pv. tabaci IFO-3508 (minimum inhibitory concentration (MIC) : 0.4 μg/ml), and also showed antifungal activity against six kinds of Fusarium oxysporum species (MIC : 0.1-2.0 μg/ml). The antimicrobial activity of RM-B was much stronger than those of RM-A and -C which contain, respectively, one and two β-lysine moieties in their molecules. The above activities of RM-A, -C and -B were thus in the order of -B>-C>-A : namely, the biological activity of racemomycin compounds tended to be stronger with increase in number of β-lysine moieties in the molecule.抄録全体を表示PDF形式でダウンロード (478K) -
辻坊 裕, 坂本 隆志, 宮本 勝城, 草野 源次郎, 小倉 勝, 長谷川 徹, 稲森 善彦1990 年 38 巻 8 号 p. 2299-2300
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーAn alkalophilic actinomycete, strain OPC-533 regarded as Nocardiopsis dassonvillei subsp. prasina, produced the cytotoxic substance, TS-1, which showed a marked inhibitory activity against L5178Y mouse leukemic cell in vitro. The cytotoxicity of TS-1 on this cell was very strong and its ID50 was 0.018 μg/ml. Through direct comparison of its spectral data with those of an authentic sample, TS-1 was identified as the antifungal antibiotic, kalafungin, already isolated from the culture broth of Streptomyces tanashiensis. However, the isolation of kalafungin from an alkalophilic actinomycete and its cytotoxicity are reported for the first time in this paper.抄録全体を表示PDF形式でダウンロード (487K) -
安孫子 敬, 関野 宏1990 年 38 巻 8 号 p. 2301-2304
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーA thymosin β4-like peptide, thymosin βMet9 isolated from pork spleen, was synthesized using a conventional solution method. The deprotection of the protected thymosin βMet9 was achieved by treatment with Zn-90% AcOH to remove C-terminal ρ-nitrobenzyl ester and then with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio, 1 : 1) in trifluoroacetic acid in the presence of dimethylselenium. Finally, the deprotected peptide was incubated with dithiothreitol to reduce sulfoxide on the methionine side chain.The increase of the E-rosette-forming lymphocytes was obtained after incubation of peripheral blood from lupus nephritis patients with the synthetic thymosin βMet9. The synthetic thymosin βMet9 was approximately equal in potency to that of our synthetic calf thymosin β9.抄録全体を表示PDF形式でダウンロード (748K) -
山口 正義, 森 聖一1990 年 38 巻 8 号 p. 2305-2307
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーThe effect of heparin on the calcium-binding protein regucalcin-stimulated Ca2+ release from rat liver microsomes was investigated. Ca2+ release was assayed by the method of Millipore filtration to estimate microsomal 45Ca2+ accumulation following the addition of 10 mM adenosine triphosphate. The addition of regucalcin (1.0 μM) or inositol 1, 4, 5-trisphosphate [Ins(1, 4, 5)P3; 1.0 μM] stimulated 45Ca2+ release from rat liver microsomes. These effects were completely inhibited by the presence of heparin (10.0 μg/ml). Regucalcin did not enhance the effect of Ins(1, 4, 5)P3. These results suggest that regucalcin affects 45Ca2+ release involved in Ins(1, 4, 5)P3 action in rat liver microsomes.抄録全体を表示PDF形式でダウンロード (502K) -
佐々木 均, 児島 正樹, 中村 純三, 柴崎 寿一郎1990 年 38 巻 8 号 p. 2308-2310
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーWe investigated preliminary acute toxicity and primary skin irritation of nine pyrrolidone derivatives which had been previously developed as transdermal penetration enhancers. The acute toxicity was observed at a dose of 500 mg/kg after intraperitoneal administration in mice. Their primary skin irritations were examined with rabbit dorsal skin. 1-Lauryl-2-pyrrolidone induced the most severe irritation among the derivatives. Pyrrolidone derivatives having methyl group and methyloxycarbonyl group caused little irritation. The primary irritation indices of pyrrolidone derivatives were not relative to their accumulations in the skin but to their enhancing effects. In conclusion, 1-hexyl-4-methyloxycarbonyl- and 1-lauryl-4-methyloxycarbonyl-2-pyrrolidone are suggested to be adequate enhancers, judging from the balance of their enhancing activity and irritation.抄録全体を表示PDF形式でダウンロード (517K) -
小泉 保, 稲田 裕彦1990 年 38 巻 8 号 p. 2311-2313
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーThe time-dependent theoretical solution to ionic mass transport through a uniform membrane under the influence of a uniform electric field is derived, satisfying the initial condition of diffusional (non-iontophoretic) steady state. With increasing voltage magnitude, the lag times increase from zero, to attain the maximum and decrease to zero.抄録全体を表示PDF形式でダウンロード (444K) -
山口 正俊, 丹野 慶紀, 杉本 堅次, 森本 雍憲1990 年 38 巻 8 号 p. 2314-2316
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーAttempts were made to investigate the disintegration test for vaginal tablets. Disintegration tests were done for four different commercial vaginal tablets (three lots each) by the watch glass method and Japanese Pharmacopoeia (JP) disintegration method, and the resulting profiles were compared to those by the modified British Pharmacopoeia (BP) method on a point of lot-to-lot variation of the disintegration times. The disintegration time of every tablet by the modified BP method was longest, followed by the watch glass method, and finally by the JP disintegration method. The results for lot-to-lot differences in disintegration times by the modified BP method were similar to those by the watch glass method. However, such lot-to-lot differences as found by the modified BP method and watch glass method were not always observed by the JP desintegration method. It was concluded from these results that the modified BP method was most suitable for investigating lot-to-lot differences in the disintegration of vaginal tablets.抄録全体を表示PDF形式でダウンロード (488K) -
渡辺 和人, 木島 貴子, 成松 鎮雄, 西上 潤, 山本 郁男, 吉村 英敏1990 年 38 巻 8 号 p. 2317-2319
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーPharmacological effects (catalepsy, hypothermia, pentobabrital-induced sleep prolongation, anticonvulsant and analgesic effects) of Δ8- and Δ9-tetrahydrocannabinols, and their 11-hydroxy-metabolites were evaluated and compared in mice Δ9-Tetrahydrocannabinol and 11-hydroxy-Δ9-tetrahydrocannabinol exhibited somewhat greater effects than did Δ8-tetrahydrocannabinol and 11-hydroxy-Δ8-tetrahydrocannabinol, respectively, in all pharmacological indices tested. Greater effects of 11-hydroxy-metabolites than those of tetrahydrocannabinols were also demonstrated.抄録全体を表示PDF形式でダウンロード (530K) -
塩本 秀己, 松田 秀秋, 久保 道徳1990 年 38 巻 8 号 p. 2320-2322
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーThe mode of action of protopine on rabbit platelet aggregation was investigated in the metabolic system of adenosine 3', 5'-cyclic monophosphate (cyclic AMP) in vitro experimental models.The inhibitory activity of protopine on adenosine 5'-diphosphate induced platelet aggregation was increased in the presence of prostaglandin I2 or papaverine in platelets. Protopine elevated content of the basal cyclic AMP accumulation in platelets and enhanced activity of crude adenylate cyclase prepared from platelets, but was ineffective on cyclic AMP phosphodiesterase.It is concluded that protopine has an inhibitory activity on platelet aggregation, activates adenylate cyclase and increases cyclic AMP content in platelets, in addition to other inhibitory actions in the metabolic system of cyclic AMP.抄録全体を表示PDF形式でダウンロード (588K) -
橋本 俊一, 本田 雄, 池上 四郎1990 年 38 巻 8 号 p. 2323-2325
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーAn efficient 1, 2-trans-glycosidation reaction withon neighbouring group participation has been developed using benzyl-protected P, P-diphenyl-N-(p-toluensulfonyl)phosphinimidates as glycosyl donors in the presence of trimethylsilyl triflate or boron trifluoride etherate.抄録全体を表示PDF形式でダウンロード (472K) -
木内 文之, 劉 宏民, 津田 喜典1990 年 38 巻 8 号 p. 2326-2328
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーTwo new saponins, gymnemic acid-VIII and -IX, were isolated from the water extract of leaves of Gymnema sylvestre R. Br. along with three known saponins, gymnemic acid III, IV, and V. Their structures, clarified on the basis of spectroscopic and chemical means, present rare examples of a hexulopyranoside moiety at 0-3' on gymnemic acid-III and -IV, respectively.抄録全体を表示PDF形式でダウンロード (406K) -
村田 正和, 生駒 繁一, 阿知波 一雄1990 年 38 巻 8 号 p. 2329-2331
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーThe asymmetric synthesis of chiral cis-endo-5-norbornene-2, 3-dimethanol mono acetate by lipase-catalyzed transesterification, and it was used to synthesize an optically active TXA2 antagonist.抄録全体を表示PDF形式でダウンロード (384K) -
和田 啓爾, 八木 昌明, 松村 章弘, 佐々木 啓子, 阪田 正勝, 羽賀 正信1990 年 38 巻 8 号 p. 2332-2334
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーPhellodendri cortex and its components, limonin and obacunone, shortened the sleeping time induced by α-chloralose and urethane (50 and 500 mg/kg respectively, intraperitoneal route) in mice.抄録全体を表示PDF形式でダウンロード (382K) -
千葉 卓男, 高田 芳宏, 内藤 俊彦, 金子 主税1990 年 38 巻 8 号 p. 2335-2337
発行日: 1990/08/25
公開日: 2008/03/31
ジャーナル フリーA novel method is described for introducing a two-carbon unit at either the 3- or 4-position of the 1-isoquinolone ring. The method for the synthesis of the 4-vinyl or 4-ethynyl derivative of isoquinolone consists of 1) photo-addition of the isoquinolone to chloroethylenes and 2) base treatment of the major (head-to-tail) adduct giving the 4-substituted isoquinolones. Similarly, by using 3-methoxyisoquinolone and vinyl acetate in step 1) and an acidic condition in step 2), a formylmethyl group is introduced into the 3-position of the isoquinolone ring.抄録全体を表示PDF形式でダウンロード (407K)