巻号一覧
74 巻, 6 号
選択された号の論文の13件中1～13を表示しています
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Junji ITO1998 年 74 巻 6 号 p. 199-205
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーThe muscle fiber composition and cross-sectional area of muscle fiber types were investigated histochemically in the abdominal muscles (rectus abdominis muscle, obliquus externus abdominis muscle, obliquus internus abdominis muscle and transversus abdominis muscle) of three Japanese macaques (Macaca fuscata). Muscle fibers were classified into three fiber types (Type I, II A and II B) by myosin ATPase activity and succinate dehydrogenase activity. Each abdominal musde in Japanese macaques contained high proportion of Type II B fibers and there were no large differences in the fiber type composition between the abdominal muscles. The range of mean fiber type percent was 26-32%Type I,21-22% Type II A, and 46-52% Type II B fibers. Thus, based on the histochemical fiber type composition, the separate abdominal musdes appear to have a similar functional capacity. The cross-sectional area was larger for Type II than for Type I fibers, and the areas were similar in Type II A and Type II B fibers in each muscle. The rectus abdominis showed larger fibers of each type compared to the lateral abdominal muscles. The high proportion of Type II B fibers and large fiber size for Type II B fibers suggest that the abdominal muscles of Japanese macaques have properties similar to the propulsive and locomotory muscles in the limbs.抄録全体を表示PDF形式でダウンロード (1462K) -
Keiichi MORIGUCHI, Michiya UTSUMI, Norikazu OHNO1998 年 74 巻 6 号 p. 207-215
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーWe examined cell fixation with microwave irradiation (MWI) used in cytochemistry. MWI was applied to blocks of about 1mm3 of mouse parotid glands at 500W for about 5 sec in a fixative at 37°C. The activities of endogenous peroxidase and mitochondrial cytochrome oxidse were demonstrated by usinng the DAB method with 3,3'diaminobenzidine (DAB) and 0.01% H2O2. Under electron microscopy, peroxidase activity was localized in the nuclear envelope, endoplasmic reticulum and sectetory granules. However, mitochondria cytochrome oxidase activity seemed to be rather weak against the MWI at 37°C. Moreover, suspension of isolated hamster liver mitochondria was fixed by MWI and also demonstrated cytochrome oxidase activity by using the cytochemical methods with DAB, cytochrome c, catalase and sucrose. Such mitochondrial fractions were subjected to 6-second MWI given 10 or 18 times with an interval of 10 seconds with and without a chilled water bath. The final temperature of each fixative was kept at about 10°Cor rose to about 37 and 55°C. When we took care to keep the temperature below 10°C, the DAB reaction products accumulated in the mitochondrial intermembrane-intracristal space. No mitochondrial deposits were observed when the temperatures of the fixatives rose to 37 and 55°C. These results indicated that peroxidase was very resistant to the heat with MWI fixation. Cytochrome oxidase is sensitive to the heat with MWI, so, a chilled water bath had to be used.抄録全体を表示PDF形式でダウンロード (2534K) -
Noriaki SATO, Toshivuki MORITA, Isamu ISHIWATA, Mitsuo NAKAI, Koji KAM ...1998 年 74 巻 6 号 p. 217-229
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーCellular characterization of the 91kDa-ectopic ascitic protein that exhibits pregnancy-associated and tumourrelated dynamics has been examined in the human placenta using an electron microscopic immunocolloidal-gold technique. This protein was initially isolated from the ascitic fluids of a patient suffered from ovarian and uterine cancers with mixed mesodermal tumours, and determined to be sharing antigenicity with the 28kDa-oncodevelopmental protein and a calcium-binding protein; MRP8/CFA, respectively. Placentas obtained were divided into three groups by their gestational periods. Small chorionic villous tissues were embedded in Lowicryl K4M resin or Epon 812 resin. Specific and higher labellings by gold-particles were obtained in sections of Lowicryl resin and, then, recognized in mesenchymederived cells and/or myeloid lineages; such as placental tissue macrophages (Hofbauer cells), fibroblasts, foetal myelomonocytic cells including endothelial cells, etc., in the first and second trimesters. So far, the pattern of antigenic appearances changed depending on the stage of gestation. On the other hand,91kDa-protein was also determined in the syncytiotrohpoblast, but not in cytotrophoblasts at whenever been examined. It is assumed that the antigenic expression in syncytiotrophoblasts might be reflected to be absorbed or incorporated from those of foetal or maternal origins, and the antibody used in this study should be sensitive to the antigenic epitope derived from those of myeloid lineages. In the light of these results, hypotheses concerning mechanisms of both transplacental permeability of substances by the placental barrier and cell/tissue differentiation by calcium-binding (and/or -depending) proteins such as 91kDa-protein, MRP8, etc.; presumable the S-100 protein family, are discussed further.抄録全体を表示PDF形式でダウンロード (4229K) -
Akio HIURA, Fumio NASU, Hiroshi ISHIZUKA1998 年 74 巻 6 号 p. 231-235
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーThe synaptic relationships between primary afferent central endings containing substance P (SP) and calcitonin gene-related peptide (CGRP) and GABAergic internenrons in the guinea pig substantia gelatinosa of the lumbar spinal dorsal horn were studied. The pre-embedding PAP method was used for detection of GABA and the post-embedding double immunogold labeling method for SP and CGRP detection. Immunogold particles specific for SP and CGRP were mainly localized separately or together in large synaptic vesicles devoid of dense cores. SP and CGRP immunoreactivity was separate or co-localized in small roundish, slender, sinuous or large scalloped (fan-like) terminals with closely packed round agranular synaptic vesides of various sizes and few large dense core vesides and mitochondria, which are thought to be capsaicin-sensitive primary afferent terminals. These SP-and CGRP-immunoreactive boutons make presynaptic or symmetric contacts with GABAergic dendrites and soma. These findings suggest that the central endings of nodceptive primary afferents transmit pain stimuli to intrinsic inhibitory internenrons, thereby modulating nodceptive information via a postsynaptic circuit.抄録全体を表示PDF形式でダウンロード (1986K) -
Shintaro KONDO, Hajime HANAMURA, Masahito NATORI, Eizo WAKATSUKI1998 年 74 巻 6 号 p. 237-242
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーThe maxillary first and second molars (M1 and M2) in the Japanese shrew mole, Urotrichus talpoides, were investigated using an odontometrical approach. The mesiodistal crown diameter was larger in M1 than in M2, while the buccolingual diameter of M1 was nearly equal to that of M2. M2 was more compressed mesiodistally than M1. M1 had a large distal triangle on the stylar shelf. The mesial triangle of M2 was slightly larger than the distal triangle. Despite being smaller than M1, M2 was less variable than M1 in terms of size. The distal triangle of M1 and the mesial triangle of M2 were well developed, and thus this area, which corresponds to the inflection point of the maxillary dental arch, was most likely the center of an occlusal function.抄録全体を表示PDF形式でダウンロード (1390K) -
Tamotsu SAITO, Gen MURAKAMI1998 年 74 巻 6 号 p. 243-257
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーOne thousand and thirty eight dorsal cutaneous vessels, which emerged onto the superficial fascia behind the thoracic vertebrae, were examined with regard to the point at which they perforated the fascia, their diameters, intermuscular courses and origins. Large vessels (more than 1 mm in diameter) were mainly distributed at the T1-T4 level. The intermuscular courses could be classified into three groupes: a descending scapular course arising from the tranverse cervical vessels (4.0%), a nerve-accompanying vertebral course (68.6%) and a deep vertebral course arising from other origins (27.4%). Vessels with a nerve-accompanying course ran through the middle sized (3-4 vertebral segments long) semispinalis muscle slips and descended over 1-2 vertebral segments. Vessels with a deep vertebral course, including the posterior external vertebral venous plexus, were located around the rotatores muscle. Vessels with a deep vertebral course as well as those following a nerve-accompanying course frequently emerged onto the fascia with a nerve since the former merged into the nerve-accompanying vessels immediately before perforating the fascia. The potential clinical applications of these results are discussed抄録全体を表示PDF形式でダウンロード (2616K) -
Akira TAMADA, Shoichi EMURA, Daisuke HAYAKAWA, Huayue CHEN, Marjan JAM ...1998 年 74 巻 6 号 p. 259-269
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーTo investigate the morphological changes of the parathyroid gland of the immobilized hamsters, we studied the ultrastructure of the parathyroid gland of golden hamsters kept in special small cage Mailman cage II). All hamsters of the control group were kept in one ordinary cage. Each hamster of the isolated group was kept in ordinary cage individually. Each hamster of the immobilized group was kept in Ballman cage II individually. All hamsters were kept for 5 days. On the first and fifth day of the experiment, bone mineral content (BMC) and bone mineral density (BMD)of whole body were measured by dual energy X-ray absorptiometry (DXA). In the control and isolated groups, BMD of the fifth day was significantly increased as compared to that of the first day. In the immobilized group BMC and body weight were significantly decreased. There was no significant difference among 3 groups concerning the mean serum calcium level. Volume density of the cell organelles and indusions was estimated and compared among 3 groups. Volume density of the lysosomes and large vacuolar bodies of the isolated and immobilized groups was significantly higher than that of the control group. Much more lipid droplets were observed in the immobilized group than the control and isolated groups. No particular differences were observed as to the Golgi complex in the isolated and the immobilized groups compared to the control group. These findings suggest that the cellular activity of the parathyroid gland is suppressed with immobilization.抄録全体を表示PDF形式でダウンロード (4167K) -
Rumio TAGA, Luciano BONATELLI BISPO, Rodrigo AYRES BORDIN, Renato MASS ...1998 年 74 巻 6 号 p. 271-278
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーPostnatal mouse pancreas growth was submitted to allometric analysis by the Wald nonparameiric method, modified by Bartlett. The body and gland mass were obtained and the total cell number and the absolute compartmental volumes of adni, ducts and stroma were evaluated by morphometric methods. The allometric coefficients were calculated for the growths between the following parameters: a) pancreatic mass and body mass; b) acinar compartmental volume and body mass; c) ductal compartmental volume and body mass; d) stroma volume and body mass; e) total cell number and body mass; and f) minor volume and stroma volume. The results of these analysis showed that the pancreatic mass, total cell number and stroma volume exhibited statistically significant allometric growths with a monophasic pattern and allometric coefficients of 1.56,1.27 and 1.29, respectively, for the periods of 2 to 70,2 to 28 and 2 to 70 days of age; while the growth of compartmental acinar volume in relation to body mass and compartmental stroma volume was biphasic. In the first case, the 1st phase occurred between 2 to 14 days (K = 1.09) nd the 2nd phase between 14 to 70 days (K = 1.44) and in the second case, the 1st and 2nd phases occurred, respectively, between 2 to 28days (K = 1.31) and 28 to 70 days (K = 0.79) of age. The growth of ductal volume in relation to body mass was also biphasic with a 1st phase between 2 to 14 days (K = 0.88) and a 2nd phase between 14 to 70 days (K = 1.07). These results permitted us to condude that the growth of the mouse pancreas is allometrically associated with the growth of body mass.抄録全体を表示PDF形式でダウンロード (1780K) -
B. H. BAY, M. C. W. WANG, G. W. C. YIP1998 年 74 巻 6 号 p. 279-291
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーIntraperitoneal administration of zinc chloride in vivo at 14 μg/g body weight (equivalent to 1/2 LD50) over a 3 week period induced an increase in liver weight. The mean weight of the livers in zinc-treated C57/6J mice was 50%higher than that of control animals. Image analysis revealed a concomitant and significant increase in the cross-sectional area and perimeter of the hepatocytes in the zinc-treated group. The mean cross-sectional area of hepatocytes in the zinc-treated mice was 337.2±4.3 μm2 as compared to 224.6±2.9 μm2 in the control group (p<0.05, Mann-Whitney test). The mean perimeter, of hepatocytes in zinc-treated mice was 72.4±0.5 μm as compared to 58.9±0.4 μm in control animals (p<0.05). It would appear that subacute administration of zinc may result in increase in liver mass due to hypertrophy of the hepatocytes.抄録全体を表示PDF形式でダウンロード (1315K) -
GÜRSOY Erdogan, BULUT Hüseyin Eray, BASIMOGLU-KOCA Yucel1998 年 74 巻 6 号 p. 293-315
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーVery complex cellular events take place at the morphological and molecular levels both in the maternal and embryonic sites during blastocyst implantation. Although the morphological alterations in the gestational stages of the implantation site are well-known, mechanisms of the interaction between blastocyst and endometrial epithelium (apposition and adhesion stages) and penetration of the blastocyst into the endometrium have not yet been fully described. Therefore the present study was designed to determine these events morphologically throughout the implantational stages. To do this the endometrial tissues containing implantation sites from pregnant rats were taken 5 and 8 days after fertilization. In addition, non-pregnant rat endometrial tissues were also obtained in order to use as control. Tissues were processed for light and electron microscopy. Semi-thin and ultra-thin sections were stained using appropriate staining methods, and were evaluated under light and electron microscope. In the present study, interaction between trophoblast and luminal epithelium, penetration of the blastocyst into the endometrium, differentiation of germinal layers in the blastocyst and tissue alterations such as decidualization in the maternal site have been demonstrated morphologically. In condusion, while blastocyst was implanted into the endometrium, distinct morphological changes occurred both in maternal and embryonic sites. The following stages of implantation were the development of germinal layers in the embryo and morphological changes in the maternal site leading to the placentation.抄録全体を表示PDF形式でダウンロード (4788K) -
Yuuki OGITA, Yasutomo IWAI-LIAO, Yoshikage HIGASHI1998 年 74 巻 6 号 p. 317-327
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーTopographic and tomographic studies were conducted on the organic elements occluded in the enamel of premolars removed from young orthodontic patients by using light (transmitted) microscopy, confocal scanning laser microscopy (CLSM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) on ultrathin sections and freeze-etching replicas, and energy dispersive spectroscopy (EDS) X-ray microscope (EDX) analysis. The present fine structure study aimed in particular to determine the fine structure of the enamel spindle and the extent of the odontoblast process. Organic elements in the ground-sectioned enamel corresponding to simple projections and enamel rods/spindles, enamel tufts and lamellae were identified by conventional light microscopy and subsequently examined by CLSM. Both light microscopy and CLSM indicated that a number of enamel spindles were measured about 50 μm in length, some 4-7 μm in thickness and were mostly confined to the cuspal summits and conformed to previous descriptions. SEM examination revealed some simple projections extending from the dentine into the enamel as well as clearly identifiable enamel spindles; the enamel spindles were structures intervening enamel prisms and showing morphological complexity by branching and convergence of the distal endings of the invading organic structure from dentinal tubules. EDXanalysis revealed that enamel tufts, lamellae, and spindles contained less phosphorus and calcium elements than enamel prisms. The enamel spindles had a higher content than tufts or lamellae, but this may be the result of contamination from surrounding enamel. Both conventional ultrathin-section and freeze-etching replica TEM evaluation of the dentino-enamel boundaries in particular suggested that simple projections and enamel rods/spindles were extensions of the odontoblast processes trapped in the enamel during early amelogenesis. In contrast, both SEM and TEM observations failed to identify dentinal tubule, peritubular (intratubular) dentine, membranous structures or lamina limitans surrounding the enamel spindles and simple projections ocduded in the human enamel.抄録全体を表示PDF形式でダウンロード (3598K) -
Nobusuke KOBAYASHI, Yoshiharu SAWABE, Kiyoshi MATSUMOTO, Naruhito OTSU ...1998 年 74 巻 6 号 p. 329-335
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーUnmyelinated nerve fibers of the abducent nerve have occasionally been observed with electron microscopes, but, to our knowledge, they have never been evaluated from the morphometric point of view. We analyzed the aging process of the unmyelinated nerve fibers in the human abducent nerve with the help of a new staining method suitable for morphometric research on the nervous system. We studied numbers and transverse areas of unmyelinated fibers of the abducent nerve in 10 cadavers. Our findings were that (1) these fibers were distributed diffusely, (2) their number decreased with age, and (3) the mean transverse area did not change with age. Most of the unmyelinated axons were thinner than the myelinated axons. These results may be important for analysis of clinical signs in relation to aging and ophthalmologic functions.抄録全体を表示PDF形式でダウンロード (790K) -
Yoshiharu SAWABE, Kiyoshi MATSUMOTO, Noboru GOTO, Naruhito OTSUKA, Nob ...1998 年 74 巻 6 号 p. 337-343
発行日: 1998/03/20
公開日: 2012/09/24
ジャーナル フリーMyelinated nerve fibers of the human abducent nerve were analyzed with a new staining method that permits simultaneous observation of the axon and surrounding myelin sheath. The following equipment was employed for the measurements: an image-analyzing digitizer, a microscope equipped with a drawing tube (or camera lucida), and a computer for data storage and statistical analysis. The numbers, transverse areas, and circularity ratios of axons were measured in 10 human abducent nerves. The average number was 1,997 with a definite decrease with age, and the average area was 3.90 μm2抄録全体を表示PDF形式でダウンロード (1024K)
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