巻号一覧
86 巻 (2009)
- 4 号 p. 111-
- 3 号 p. 73-
- 2 号 p. 37-
- 1 号 p. 1-
86 巻, 3 号
選択された号の論文の5件中1～5を表示しています
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Gen NIIMI, Koji OHASHI, Tomihiko IDE, Jaime PEREDA2009 年 86 巻 3 号 p. 73-77
発行日: 2009年
公開日: 2010/02/12
ジャーナル フリーThis paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.抄録全体を表示PDF形式でダウンロード (4148K) -
Alimujiang SHAWUTI, Takayoshi MIYAKI, Toshiyuki SAITO, Masahiro ITOH2009 年 86 巻 3 号 p. 79-88
発行日: 2009年
公開日: 2010/02/12
ジャーナル フリーTo get the full understanding of the arterial distribution to the pancreas, the analysis of the distribution of the variety of monkey species would be helpful. In this study, we studied the layout of the pancreatic artery in anthropoids (1 gorilla, 3 chimpanzees and 2 white-handed gibbons), in catarrhine monkeys (1 hamadryas baboon, 2 anubid baboons, 10 savannah monkeys) and in platyrrhine monkeys (6 squirrel monkeys).
The pancreas of the monkeys was supplied by the arteries originating from the celiac trunk and/or superior mesenteric artery. There were three patterns in the arterial distribution; (1) the celiac artery supplied the major area of the pancreas. (2) the superior mesenteric artery supplied the major area of the pancreas. (3) the celiac artery supplied the whole pancreas. The pattern of the arterial distribution to the monkey pancreas had a wide variety. The result would be helpful for the elucidation of the development of the vascular distribution in the pancreas.抄録全体を表示PDF形式でダウンロード (1559K) -
Masataka SUNOHARA, Shigeru MORIKAWA, Hidetaka MURATA, Akira FUSE, Iwao ...2009 年 86 巻 3 号 p. 89-91
発行日: 2009年
公開日: 2010/02/12
ジャーナル フリーThrombopoietin receptor (c-Mpl) is considered to regulate megakaryocytopoiesis. In this study, we investigated an effect of activation of a protein kinase C (PKC) on c-mpl promoter activity to elucidate the underlying mechanisms of c-mpl gene expression in megakaryoblastic cells. PKC is a member of a family of serine/threonine protein kinases in the cytosol involved in cell growth and differentiation. Phorbol 12-myristate 13-acetate (PMA) is known as PKC activator, significantly enhanced the c-mpl promoter activity and PKC inhibitors (H7, GF109203) suppressed the up-regulation of PMA-induced promoter activity and reduced the steady level of its activity. These results strongly suggest that PKC plays the essential role in the modulation of c-mpl promoter activity of megakaryoblastic cells.抄録全体を表示PDF形式でダウンロード (734K) -
Megumi MURATA, Yoko MIWA, Iwao SATO2009 年 86 巻 3 号 p. 93-103
発行日: 2009年
公開日: 2010/02/12
ジャーナル フリーThe activity of respiratory chain enzymes in a rat’s masseter muscle changes as the animal ages; however, there is little information about the RNA transcript levels of mitochondrial enzymes in klotho mutant mice as they age. We measured the activities of NADH-ferricyanide oxidoreductase and NADH-O2 oxidoreductase, and the RNA transcript levels of NADH dehydrogenase, the mitochondrial isoform of ND1, the nuclear isoforms of the 51 kDa and 75 kDa subunits of Complex I, the nuclear isoform of cytochrome c, and the mitochondrial isoform of beta subunits of ATPase (Complex V). In addition, we measured the RNA transcript levels of catalase (CAT) and superoxide dismutase (SOD), which are associated with antioxidant proteins. Moreover, we measured ATP concentrations using a luciferin-luciferase assay, and we determined the amount of cytochrome c associated with mitochondria in both klotho mutant mice and wild-type mice. However, the mRNA levels of cytochrome c and Complex V components, the mRNA levels of CAT, SOD, and apoptosis-inducing factor (Aifm), and the protein level of cytochrome c remained constant as klotho mutant mice aged from 5 weeks to 7 weeks. In wild-type mice, these components (except for those of Complex I) increased over time. NADH-ferricyanide oxidoreductase and NADH-O2 oxidoreductase activities decreased in klotho mutant mice as they aged from 5 weeks to 7 weeks. A few large mitochondria were scattered between myofibrils, and 7-week-old klotho mutant mice displayed an increased number of irregular mitochondria with fewer cristae. Our results indicate that the klotho protein plays a role in the diminished functional adaptability of enzymes in the masseter muscle of klotho mutant mice throughout the aging process.抄録全体を表示PDF形式でダウンロード (1484K) -
Yoko MIWA, Masataka SUNOHARA, Iwao SATO2009 年 86 巻 3 号 p. 105-110
発行日: 2009年
公開日: 2010/02/12
ジャーナル フリーWe investigated the properties of the masseter muscle in mice from five to seven weeks of age. Myosin heavy chain (MyHC) isoforms were measured in the masseter muscle. The three types of muscle fibers (Type I, strong reaction; Type IIA, intermediate reaction; and Type IIB, weak reaction) were all present in the masseter muscle in five-weeks-old mice and seven-weeks-old mice, the three types could be clearly distinguished by their enzyme activity. The percentage of Type IIB fibers (above 50%) was the highest among all fiber types both 5- and 7-weeks-old mice. The mRNA levels for myosin slow and myosin IIb increased significantly between 5 and 7 weeks. These observations suggest that muscle fiber size, muscle fiber types and mRNA levels of the MyHC isoforms all contribute to the diminished functional adaptability of enzyme activity in the masseter muscle.抄録全体を表示PDF形式でダウンロード (1160K)
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