Two main coloring matters were isolated in crystalline forms from an aqueous reaction mixture of acetophenone and 1, 3, 5-trinitrobenzene under the Janovsky conditions. One of them was the Meisenheimer type compound, sodium salt of II. The other was a new compound and the structure was determined as sodium salt of diphenacyltrinitrocyclohexenide anion III (Chart 3). The spectral behavior of these coloring matters was compared with that of the reaction mixture and the color reaction of acetophenone and TNB under the aqueous Janovsky conditions was discussed.