From bulbs of Allium ampeloprasum L. (elephant garlic : Liliaceae), a new spirostane-type saponin, named ampeloside Bs₁ (7), and two new furostane-type saponins, named ampelosides-Bf₁ (8) and -Bf₂ (9), were isolated along with a known spirostane-type saponin, the prosapogenin of aginoside (1). The structures of the new saponins (7-9) were established as agigenin 3-O-β-glucopyranosyl(1→3)-β-glucopyranosyl(1→4)-β-galactopyranoside, (25R)-26-O-β-glucopyranosyl-22-hydroxy-5α-furostane-2α, 3β, 6β, 26-tetraol-3-O-β-glucopyranosyl(1→3)-β-glucopyranosyl-(1→4)-β-galactopyranoside and (25R)-26-O-β-glucopyranosyl-22-hydroxy-5α-furostane-2α, 3β, 6β, 26-tetraol-3-O-β-glucopyranosyl(1→4)-β-galactopyranoside, respectively. Antifungal activity of these saponins (1, 7 and 8) is discussed.