In connection with our studies on the chemical evaluation of commercial Bupleuri Radix, isolation and identification of saponins of Juk-Siho (a Korean Bupleuri Radix) and roots of Bupleurum longeradiatum, the source plant of this crude drug, were carried out. From Juk-Siho, five saponins 1-5 were isolated. The saponins 1-3 were identified as saikosaponins-a (1), -c (2) and -d (3), respectively, all of which have already been isolated from roots of Bupleurum falcatum. The structures of the new saponins, named chikusaikosides-I (4) and -II (5), were estabished as 3-O-β-xylopyranosyl (1→2)-β-glucopyranosyl-(1→3)-β-fucopyranoside of saikogenin F (6) and 3-O-β-glucopyranosyl (1→6)-(α-rhamnopyranosyl (1→4)-β-glucopyranoside of 6, respectively. From roots of B. longeradiatum, saponins 1-4 were isolated and identified. During the course of the structure study of 4 by ¹³C-nuclear magnetic resonance (¹³C-NMR), an anomalous glycosylation shift for the 1, 2-linked glycoside was encountered.