Sugar parts play important roles in recognizing molecules on the cell membranes. We successfully produced sugar-type micellar surfactants, lactonoalkylamide (LacC_n), for the first time. Spherical vesicles, three-component hybrid liposomes, were obtained after the sonication of the mixture of L-α-dimyristoylphosphatidylcholine (DMPC), Tween 20 and LacC_n (DMPC : Tween 20 : LacC_n=65 : 7 : 28). It is noteworthy that high inhibitory effects of the three-component hybrid liposomes composed of DMPC, Tween 20, and LacC_n (DMPC : Tween 20 : LacC_n=65 : 7 : 28) on the growth of glioma (U251) and lung adenocarcinoma (RERF-LC-OK) cells were attained in vitro without any drug, although no significant inhibitory effects of any individual component (DMPC, Tween 20, LacC_n) or the two-component hybrid liposomes of DMPC and Tween 20 on the growth of tumor cells examined were obtained.